Isolation and characterization of kinetoplast DNA networks and minicircles from Crithidia fasciculata.
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چکیده
7. Cook, J. R. 1968. The cultivation and growth of Euglena, in Buetow, D. E., ed., The Biology of Euglena, Academic Press, New York 1, 243-314. 8. Cooper, T. G. & Beevers, H. 1969. P-oxidation in glyoxysomes from castor bean endosperm. J . Biol. Chem. 244, 3514-20. 9. Cramer, M. & Myers, J. 1952. Growth and photosynthetic characteristics of Euglena gracilis. Arch. Mikrobiol. 17, 384-402. 10. Danforth, W. F. 1968. Respiration, in Buetow, D. E., ed., The Biology of Euglena, Academic Press, New York 2, 55-71. 1 1 . De Duve, C. 1969. Evolution of the peroxisome. Ann. N .Y . Acad. Sci. 168, 369-81. 12. -& Baudhuin, P. 1966. Peroxisomes (microbodies and related particles). Physiol. Rev. 46, 323-57. 13. Frederick, S. E., Gruber, P. J. & Tolbert, N. E. 1973. The occurrence of glycolate dehydrogenase and glycolate oxidase in green plants. Plant Physiol. 52, 318-23. 14. Gomez, M. P., Harris, J. B. & Walne,‘ p. L. 1913. Ultrastructural cytochemistry of Euglena gracilis Z from aging cultures. J . Protozool. 20, 515-16. 15. Graves, L. B., Jr. 1971. Effects of different substrates on glucose uptake and hexokinase activity in Euglenu gracilis. J . Protozool. 18, 543-6. 16. -, Hanzely, L. & Trelease, R. N. 1971. The occurrence and fine structural characterization of microbodies in Euglena gracilis. Protoplasma 72, 141-52. 17. -, Trelease, R. N. & Becker. W. M. 1971. Particulate nature of glycolate dehydrogenase in Euglena : possible localization in microbodies. Biochem. Biophys. Res. Commun. 44, 280-86. 18. -, , Grill, A. & Becker, W. M. 1972. Localization of glyoxylate cycle enzymes in glyoxysomes in Euglena. J . Protozool. 19, 527-32. 19. ~ , Ackerman, K. & Becker, W. 1973. Intracellular localization of P-oxidation in Euglena. Plant Physiol. 51 (Suppl.), 18. 20. Gruber, P. J., Frederick, S. E. & Tolbert, N. E. 1974. Enzymes related to lactate metabolism in green algae and lower land plants. Plant Physiol. 53, 167-70. 21. Haigh, W. G. & Beevers, H. 1964. The occurrence and assay of isocitrate lyase in algae. Arch. Biochem. Biophys. 107,
منابع مشابه
A nicking enzyme from trypanosomatids which specifically affects the topological linking of duplex DNA circles. Purification and characterization.
Newly replicated duplex DNA minicircles of trypanosomal kinetoplast DNA are nicked in both their monomeric and catenated topological states, whereas mature ones are covalently sealed. The possibility that nicking may play a role during kinetoplast DNA replication by affecting the topological interconversions of monomeric DNA minicircles and catenane networks was studied here in vitro using Crit...
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ورودعنوان ژورنال:
- The Journal of protozoology
دوره 21 5 شماره
صفحات -
تاریخ انتشار 1974